Dear ABOL community

One advantage of DNA barcoding is the strong international networking of the initiative.

As part of the HRSM project, the team of Irmgard Krisai-Greilhuber at the University of Vienna has created DNA barcodes of a fungus that was on the way of being described.

Who does not know them from the own garden, the Spanish slug? A study at the BOKU Vienna showed that Citizen Scientists can contribute to investigate the abundance of slugs of the genus Arion in private gardens. The quality of determinations could be kept high in the study by using the DNA barcoding approach. The publication is freely available at


In keeping with the theme of a workshop held in the context of this year’s ABOL conference, a review will be devoted to the topic “Genetic methods in biological assessment of aquatic habitats”. The paper provides information on the benefits and pitfalls of (e)DNA metabarcoding approaches for calculating biotic indices, provides insights into potential future developments, and provides recommendations for the future integration of DNA metabarcoding to routine biomonitoring programs. The publication can be found at


Two publications on alpine moth species show benefits, firstly of integrative approaches, and secondly of international collaboration within the DNA barcoding community.

A comparison of representatives of the Agrotis fatidica species-group from the Alps, the Pyrenees, the Apennines and southern Norway led to the description of two new species. The publication can be found at

Among other things, a study on representatives of the genus Udea found evidence of hybridization between two species. The publication can be downloaded at

A fascinating recent publication deals with the potential of species delimitation in Lepidoptera via CO1. An international cooperation, with the participation of experts from the Tyrolean State Museums, could show that DNA barcoding is an efficient approach to determine the vast majority of European Lepidoptera.

Every determination is eased by reciprocal monophyly between species. In the absence of the latter experts are in demand to find possible explanations. The publication gives a nice overview on possible reasons for observed para- and polyphyly between species.


Mutanen, M. et al. (2016). Species-Level Para- and Polyphyly in DNA Barcode Gene Trees: Strong Operational Bias in European Lepidoptera. Systematic Biology, 65(6), 1024–1040.

Dear ABOL Community,

It was 2004 when Christian & Szeptycki revealed the Leopoldsberg to be the worldwide biodiversity hotspot of Protura, reporting 23 species from the soil of a very dry slope. Applying DNA barcoding to re-assess the diversity of these very small and blind soil dwellers at the genetic level reported 4 additional species from the same locality, one of which was new to science (Resch et al 2014).

This species named Acerentomon christiani is now formally described in a publication (Shrubovych et al. 2016) including the DNA-Barcode in its description.

A selection of scientific and popular publications on the topic of DNA barcoding in general and ABOL in particular can be accessed via the link
The main page of the Zotero literature-database displays recent additions. By activating the link „Group Library“ the structure of the database (hierarchic folders) will be opened with the entire content.

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Dear barcoding-community,
we would like to point out three papers already published in 2015 concerning Barcoding of Austrian Lepidopterae:

Early in the year P. Huemer and P.D.N Hebert presented a Barcode library containing 1489 species of butterflies from Vorarlberg. 36 species were new to the province of Vorarlberg, while two species were newly recorded for Austria.



A further study revised the taxonomy of the genus Kessleria. An integrative approach, combining morphology and DNA-Barcoding, supported the existence of 29 European species, five of these were newly described.



The third publication presented the new moth species Callisto basistrigella from the south-eastern Alps. The species can be morphologically and genetically differentiated from its sister species C. coffeella. Both species co-occur sympatrically without evidence of admixture.



During samplings in 2014, scientists of the University of Natural Resources and Life Sciences of Vienna caught several specimens of a gudgeon-like fish in the Mur River, which differentiated from sympatric Gobio obtusirostris through their large fins, elongated body shape and habitat use. Morphological studies showed that these specimens did not belong to any of the three Romanogobio species native in Austria. Preliminary DNA barcoding by the team of the ABOL Pilot study Vertebrates at the Karl-Franzens University in Graz identified the fish as a potential new species of the genus Romanogobio, as the data did not match other Eurasian gudgeons. First investigations on the range of this new species indicated that it is rather restricted and its habitat use is very similar to that of juvenile grayling. Due to its green sheen as an adult the common name emerald-gudgeon is suggested for the species.


Friedrich, T., Wieser, C., Unfer, G., Pinter, K., Daill, D., Zangl, S., & Koblmüller, S. (2015). Eine neue, unbeschriebene Gründlingsart der Gattung Romanogobio in der Oberen Mur – Eine erste Beschreibung anhand morphologischer Merkmale und DNA-Barcodes. Österreichs Fischerei, 68(4), 91–99. Cite