Sensitivity of eDNA detection of cercaria dermatitis pathogens in Upper Austrian lakes
Cercariae are infectious larvae of trematods, which develop in snails and, after hatching, infect other intermediate hosts or the final hosts. Certain cercariae (e.g. Trichobilharzia species) can cause cercaria dermatitis, also called swimmer´s itch.
The pilot study carried out in 2019 for the detection of cercaria dermatitis pathogens in Upper Austria bathing lakes by means of DNA barcoding was used to develop the basis for the genetic detection of cercariae dermatitis pathogens using the DNA barcoding approach. In the follow-up project 2020, the primers for the detection of Trichobilharzia species and thus the detection of pathogens in water samples (eDNA) are to be optimized. In addition, the temporal and spatial sensitivity of the method should be examined and DNA barcodes generated from new species.
Co-Projektleitung
Nikolaus Szucsich, Elisabeth Haring (NHM Vienna)
Team
Hubert Blatterer, Hans-Peter Grasser, Wolfgang Heinisch, Florian Billinger (Department of Water Management of Upper Austria)
Nikolaus Helmer, Christoph Hörweg, Helmut Sattmann, Luise Kruckenhauser, Michael Duda, Susanne Reier (NHM Vienna)
Kooperationspartner
Otto Eerikki Seppälä (University Innsbruck)
Petr Horak (Charles University in Prague)
Jana Bulantová (Charles University in Prague)
Project status: completed (Start: 1.1.2020)
Publication:
281293 KAB3XLVM cercaria 1 apa 50 creator asc 1 1 title 23606 https://www.abol.ac.at/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22UBJVNTX7%22%2C%22library%22%3A%7B%22id%22%3A281293%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Reier%20et%20al.%22%2C%22parsedDate%22%3A%222020-12-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BReier%2C%20S.%2C%20Haring%2C%20E.%2C%20Billinger%2C%20F.%2C%20Blatterer%2C%20H.%2C%20Duda%2C%20M.%2C%20Gorofsky%2C%20C.%2C%20Grasser%2C%20H.-P.%2C%20Heinisch%2C%20W.%2C%20H%5Cu00f6rweg%2C%20C.%2C%20Kruckenhauser%2C%20L.%2C%20Szucsich%2C%20N.%20U.%2C%20Wanka%2C%20A.%2C%20%26amp%3B%20Sattmann%2C%20H.%20%282020%29.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-ItemURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00436-020-06938-3%26%23039%3B%26gt%3BFirst%20confirmed%20record%20of%20%26lt%3Bi%26gt%3BTrichobilharzia%20franki%26lt%3B%5C%2Fi%26gt%3B%20M%5Cu00fcller%20%26amp%3B%20Kimmig%2C%201994%2C%20from%20%26lt%3Bi%26gt%3BRadix%20auricularia%26lt%3B%5C%2Fi%26gt%3B%20%28Linnaeus%2C%201758%29%20for%20Austria%26lt%3B%5C%2Fa%26gt%3B.%20%26lt%3Bi%26gt%3BParasitology%20Research%26lt%3B%5C%2Fi%26gt%3B%2C%20%26lt%3Bi%26gt%3B119%26lt%3B%5C%2Fi%26gt%3B%2812%29%2C%204135%5Cu20134141.%20%20%26lt%3Ba%20title%3D%26%23039%3BCite%20in%20RIS%20Format%26%23039%3B%20class%3D%26%23039%3Bzp-CiteRIS%26%23039%3B%20data-zp-cite%3D%26%23039%3Bapi_user_id%3D281293%26amp%3Bitem_key%3DUBJVNTX7%26%23039%3B%20href%3D%26%23039%3Bjavascript%3Avoid%280%29%3B%26%23039%3B%26gt%3BCite%26lt%3B%5C%2Fa%26gt%3B%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22First%20confirmed%20record%20of%20%3Ci%3ETrichobilharzia%20franki%3C%5C%2Fi%3E%20M%5Cu00fcller%20%26%20Kimmig%2C%201994%2C%20from%20%3Ci%3ERadix%20auricularia%3C%5C%2Fi%3E%20%28Linnaeus%2C%201758%29%20for%20Austria%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Susanne%22%2C%22lastName%22%3A%22Reier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisabeth%22%2C%22lastName%22%3A%22Haring%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florian%22%2C%22lastName%22%3A%22Billinger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hubert%22%2C%22lastName%22%3A%22Blatterer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Duda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Gorofsky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hans-Peter%22%2C%22lastName%22%3A%22Grasser%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wolfgang%22%2C%22lastName%22%3A%22Heinisch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christoph%22%2C%22lastName%22%3A%22H%5Cu00f6rweg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luise%22%2C%22lastName%22%3A%22Kruckenhauser%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nikolaus%20U.%22%2C%22lastName%22%3A%22Szucsich%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Wanka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Helmut%22%2C%22lastName%22%3A%22Sattmann%22%7D%5D%2C%22abstractNote%22%3A%22Avian%20schistosomes%20are%20of%20medical%20and%20veterinary%20importance%20as%20they%20are%20responsible%20for%20the%20annually%20occurring%20cercarial%20dermatitis%20outbreaks.%20For%20Austria%2C%20so%20far%2C%20only%20Trichobilharzia%20szidati%20Neuhaus%201952%20was%20confirmed%20on%20species%20level%20as%20causative%20agent%20of%20cercarial%20dermatitis.%20Here%20we%20present%20the%20first%20record%20of%20Trichobilharzia%20franki%20M%5Cu00fcller%20%26amp%3B%20Kimmig%201994%20in%20Austria.%20The%20species%20was%20detected%20during%20a%20survey%20of%20digenean%20trematodes%20in%20Upper%20Austrian%20water%20bodies.%20Furthermore%2C%20we%20provide%20DNA%20barcodes%20of%20T.%20franki%20as%20well%20as%20measurements%20of%20several%20parasite%20individuals%20to%20indicate%20the%20intraspecific%20diversity.%20We%20also%20recommend%20the%20usage%20of%20an%20alternative%20primer%20pair%2C%20since%20the%20%5Cu201cstandard%20COI%20primer%20pair%5Cu201d%20previously%20used%20for%20Schistosomatidae%20amplified%20an%20aberrant%20fragment%20in%20the%20sequence%20of%20T.%20franki.%20Overall%2C%20our%20study%20shows%20how%20limited%20our%20knowledge%20about%20occurrence%20and%20distribution%20of%20avian%20schistosomes%20in%20Austria%20is%20and%20how%20important%20it%20is%20to%20acquire%20such%20a%20knowledge%20to%20estimate%20ecological%20and%20epidemiological%20risks%20in%20the%20future.%22%2C%22date%22%3A%222020-12-01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1007%5C%2Fs00436-020-06938-3%22%2C%22ISSN%22%3A%221432-1955%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00436-020-06938-3%22%2C%22collections%22%3A%5B%22KAB3XLVM%22%5D%2C%22dateModified%22%3A%222021-04-09T14%3A38%3A05Z%22%7D%7D%5D%7D
Reier, S., Haring, E., Billinger, F., Blatterer, H., Duda, M., Gorofsky, C., Grasser, H.-P., Heinisch, W., Hörweg, C., Kruckenhauser, L., Szucsich, N. U., Wanka, A., & Sattmann, H. (2020). First confirmed record of Trichobilharzia franki Müller & Kimmig, 1994, from Radix auricularia (Linnaeus, 1758) for Austria. Parasitology Research, 119(12), 4135–4141. Cite
Latest News
N. FialABOL at the ÖEG Insect Camp in the Nature Park Hohe Wand14. July, 2025 - 15:39For the fourth time (in a row), ABOL participated in the 11th ÖEG Insect Camp from June 26th to 29th with a BioBlitz. This time, the trip took us to the Hohe Wand Nature Park in Lower Austria – a biogeographically highly interesting region on the northeastern edge of the Alps, where the Pannonian and […]
M. SonnleitnerABOL at the Day of Biodiversity in Baden bei Wien3. July, 2025 - 13:40On June 13 and 14, 2025, the Biosphere Reserve Wienerwald organized its 20th Day of Biodiversity– this year in the Lower Austrian part of the biosphere reserve around Baden bei Wien. Around 100 experts from various disciplines explored the diverse flora, fauna, and funga of the area from the Kurpark to the Lindkogel. In perfect […]
Days of Biodiversity ´25 – a review5. March, 2025 - 09:15The Days of Biodiversity ’25, which ABOL once again has co-organized, are over. Our thanks go to our co-organizers Biodiversity Hub, BOKU, ZooBot, NOBIS, University of Vienna and NHM Vienna for the smooth cooperation. First and foremost, however, we would like to thank all participants for inspiring lectures, interactive workshops and lively discussion rounds. Under […]
